Hematopathology Testing
HEMATOPATHOLOGY SPECIMEN REQUIREMENTS
SPECIMEN | FLOW | IMMUNOHISTO CHEMISTRY SEND CLINICAL HISTORY | HEMATOPATHOLOGY CONSULTATION SEND CLINICAL HISTORY, CBC, DIFFERENTIAL | PCR | RT-PCR( BCR-ABL, RARA ONLY) | CYTOGENEITICS CHROMOSOME FISH | SHIPPING |
---|---|---|---|---|---|---|---|
Peripheral blood | Green-, yellow- or lavender-top tube and 1 fresh smear | Not acceptable | 1 fresh smear | Lavender-top tube 5 mL (1mL minimum) | Lavender-top tube 6 mL (4mL minimum) | Green-top tube 2-5 mL | Room temperature within 48 hours |
Fresh bone marrow core biopsy | RPMI media 1 cm (length) No fixative | Place in 10% formalin | Place in 10% formalin | RPMI media 1 cm (length) | Not acceptable | RPMI media 1 cm (length) No fixative | Room temperature within 48 hours |
Bone marrow aspirate | Green-top tube 2 mL and at least 1 fresh smear | Not acceptable | At least 5 fresh smears or green-top tube 2 mL | Lavender-top tube 2-5 mL (1mL minimum) | Lavender-top tube 2-5 mL (1mL minimum) | Green-top tube 2-5 mL (1mL minimum) | Room temperature within 48 hours |
Fresh tissue biopsy | R PMI media 1 cm Place in (length) diced into fine pieces for optimal c ell preservation | 20 X 20 X 3 mm 10% formalin | 1 cm (length), minimum | Place in 10% formalin sections no thicker than 0.5 cm | Not acceptable | RPMI media 1 cm (length) | Room temperature within 48 hours |
Frozen tissue biopsy | Not acceptable | Call the laboratory | Call the laboratory | Snap frozen | Not acceptable | Not acceptable | Dry ice |
Fixed paraffin | Not acceptable | 20 X 20 X 3 mm formalin-fixed only | 0.5 X 0.5 X 0.5 cm formalin-fixed only | 0.5 X 0.5 X 0.5 cm (B5 un- acceptable) | Not acceptable | Call the laboratory | Room temperature |
Body fluids | Call the laboratory | Call the laboratory | Call the laboratory | Call the laboratory | Call the laboratory | Not acceptable | Room temperature within 48 hours |
Yellow-top tube: Acid Citrate Dextrose (ACD) solution A or B - tube must be full —Green-top tube: Sodium Heparin — Lavender-top tube: EDTA — RPMI: tissue culture media (pink) Send freshly prepared slides with the samples and a copy of the pathologist report.