Hematopathology Testing

HEMATOPATHOLOGY SPECIMEN REQUIREMENTS

SPECIMEN	FLOW	IMMUNOHISTO CHEMISTRY SEND CLINICAL HISTORY	HEMATOPATHOLOGY CONSULTATION SEND CLINICAL HISTORY, CBC, DIFFERENTIAL	PCR	RT-PCR( BCR-ABL, RARA ONLY)	CYTOGENEITICS CHROMOSOME FISH	SHIPPING
Peripheral blood	Green-, yellow- or lavender-top tube and 1 fresh smear	Not acceptable	1 fresh smear	Lavender-top tube 5 mL (1mL minimum)	Lavender-top tube 6 mL (4mL minimum)	Green-top tube 2-5 mL	Room temperature within 48 hours
Fresh bone marrow core biopsy	RPMI media 1 cm (length) No fixative	Place in 10% formalin	Place in 10% formalin	RPMI media 1 cm (length)	Not acceptable	RPMI media 1 cm (length) No fixative	Room temperature within 48 hours
Bone marrow aspirate	Green-top tube 2 mL and at least 1 fresh smear	Not acceptable	At least 5 fresh smears or green-top tube 2 mL	Lavender-top tube 2-5 mL (1mL minimum)	Lavender-top tube 2-5 mL (1mL minimum)	Green-top tube 2-5 mL (1mL minimum)	Room temperature within 48 hours
Fresh tissue biopsy	R PMI media 1 cm Place in (length) diced into fine pieces for optimal c ell preservation	20 X 20 X 3 mm 10% formalin	1 cm (length), minimum	Place in 10% formalin sections no thicker than 0.5 cm	Not acceptable	RPMI media 1 cm (length)	Room temperature within 48 hours
Frozen tissue biopsy	Not acceptable	Call the laboratory	Call the laboratory	Snap frozen	Not acceptable	Not acceptable	Dry ice
Fixed paraffin	Not acceptable	20 X 20 X 3 mm formalin-fixed only	0.5 X 0.5 X 0.5 cm formalin-fixed only	0.5 X 0.5 X 0.5 cm (B5 un- acceptable)	Not acceptable	Call the laboratory	Room temperature
Body fluids	Call the laboratory	Call the laboratory	Call the laboratory	Call the laboratory	Call the laboratory	Not acceptable	Room temperature within 48 hours

Yellow-top tube: Acid Citrate Dextrose (ACD) solution A or B - tube must be full —Green-top tube: Sodium Heparin — Lavender-top tube: EDTA — RPMI: tissue culture media (pink) Send freshly prepared slides with the samples and a copy of the pathologist report.