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QuantiFERON®-TB Gold Plus:
collection instructions

As the next generation of tuberculosis testing, the QuantiFERON®-TB Gold Plus (QFT-Plus) test employs standard phlebotomy procedures using whole blood to make sample collection easy and fast.

Test Name Test Code CPT Code
QuantiFERON®-TB Gold Plus—
1 Tube
36970(X) 86480

†The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

1-tube specimen collection and handling instructions

Specimen Collection:

  • Collect a minimum of 6 ml of blood by venipuncture into a lithium-heparin tube. Gently mix by inverting the tube several times to dissolve the heparin.
  • Hold blood drawn into lithium-heparin tube at room temperature (17–25 °C) between 15 minutesand 3 hours after blood collection.
  • Refrigerate at 2-8 °C.

Specimen Handling:

  • Place the specimen and appropriate test requisition in the QuantiFERON®-TB Gold Plus goldcollection bag.
  • Write the collection date/time on the QFT-Plus gold collection bag.
  • REFRIGERATE, DO NOT FREEZE. after at least 15 minutes at room temperature post collection. Sample must be refrigerated within 3 hours of collection.
  • If sample is left in lock box, please utilize frozen or refrigerated cold packs to maintain refrigerated sample stability. Follow standard protocol for specimens requiring refrigeration.
  • Ensure tubes are not placed in the freezer or in direct contact with frozen material. (i.e. frozen ice packs, dry ice, temperatures below 8*C, etc.)
  • Since QuantiFERON®-TB Gold Plus specimens require refrigeration, please refrain from utilizing specimen lock boxes that may be exposed to extreme temperatures (hot or cold) for long periods of time.
  • Please call in advance 866.MYQUEST (866.697.8378) in advance of specimen collection to schedule a pick-up if necessary to maintain refrigerated conditions.
  • Note the performing Quest Diagnostics laboratory must receive the specimen within 48 hours of collection under refrigerated conditions

Supply Ordering

Product ID Stock Item Number Item Description Client Will Receive
Q04 200039 QuantiFERON®-TB Gold Plus Kits, Single Tube, Lithium, 25/EA/PK 25 lithium-heparin tubes
25 collection bags

Supplies ordered through Quest Diagnostics should only be used for collection for Quest Diagnostics testing.

See important details for properly collecting specimens in the package inserts: http://www.quantiferon.com/wp-content/uploads/2017/10/QFT-Plus-ELISA-IFU-L1095849-R02.pdf.

QuantiFERON®-TB Gold Plus, 4 Tube (Draw Site Incubated)

Test Name Test Code CPT Code
QuantiFERON®-TB Gold Plus—
4 Tube (Draw Site Incubated)
36971(X) 86480

†The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

4-tube specimen collection and handling instructions

Collection:

  • Label the 4 QFT-Plus tubes appropriately
  • Tubes should be at room temperature (17°C–25°C) at the time of collection Note: specimen collection locations >3,350 feet above sea level should use the QFT-Plus collection kit designed for “high altitude” (tubes with a gold ring on tube top)
  • Note: if a butterfly needle is being used to collect blood, a “purge” tube should be used to ensure that the tubing is filled with blood prior to using the QFT-Plus blood collection tubes
  • Collect 1 mL of blood directly into each of the QFT-Plus blood collection tubes
  • As 1 mL tubes draw relatively slowly, keep the tube on the needle for 2–3 seconds once the tube appears to have completed filling
  • The black mark on the side of each tube indicates the validated range of 0.8 mL–1.2 mL. If the level of blood in any of the tubes is outside of the indicator mark, a new blood sample should be obtained
  • Shake the tubes 10 times just firmly enough to make sure the entire inner surface of the tube is coated with blood
  • STOP if you are not planning to incubate the tubes after collection

Handling:

  • Note: you have the option of sending in the sample incubated or incubated and centrifuged
  • QFT-Plus tubes must be transferred to an incubator within 16 hours of collection
  • If tubes are not incubated immediately after blood collection, re-mix tubes by inverting 10 times immediately prior to incubation
  • Incubate the 4 tubes UPRIGHT at 37°C ± 1° for 16–24 hours
  • After incubation, the 4 tubes can be centrifuged for 15 minutes at 2000–3000 RCF(G), extending stability to 28 days (refrigerated)
  • Place the specimen and appropriate test requisition in the QFT-Plus collection bag
  • Write the collection date and time on the QFT-Plus collection bag
  • REFRIGERATE specimen
  • If sample is left in lock box, please use cold packs and follow standard protocol for specimens requiring refrigeration
  • Since QFT-Plus specimens require refrigeration, please refrain from using lock boxes that may be exposed to extreme temperatures (hot or cold) for long periods of time
  • Please call 1.866.MYQUEST (1.866.697.8378) to schedule a pick-up if needed to maintain refrigeration
  • The performing Quest Diagnostics laboratory must receive the specimen within 72 hours of collection uncentrifuged and 28 days centrifuged

Additional altitude requirements

  • 2,650–3,350 feet: collect 4 mL of blood into a syringe, then immediately transfer 1 mL into each of the 4 tubes in the QFT-Plus (standard) 4 in-tube collection kit
  • 3,350–6,150 feet: collect 1 mL of whole blood directly into each of the 4 tubes in the QFT-Plus (high altitude) 4 in-tube collection kit
  • >6,150 feet: collect 4 mL of blood into a syringe, then immediately transfer 1 mL into each of the 4 tubes in the QFT-Plus (high altitude) 4 in-tube collection kit

Supply Ordering

Product ID Stock Item Number Item Description Client Will Receive
Q05 200727 QuantiFERON®-TB Gold Plus Kits, 4 Tube, 5/EA/PK Pack of 5 collection kits (kit includes the 4 tubes and a collection bag)
Q06 200728 High-altitude clients only Same as above

See important details for properly collecting specimens in the package inserts: http://www.quantiferon.com/wp-content/uploads/2017/10/QFT-Plus-ELISA-IFU-L1095849-R02.pdf.

Rely on accurate, efficient, and objective TB detection

Download the QuantiFERON-TB Gold Plus collection instructions (1 Tube)
Download the QuantiFERON-TB Gold Plus collection instructions (4 Tube)
Watch a QuantiFERON-TB Gold Plus blood collection training video
Review a QuantiFERON-TB Gold Plus resources
Contact a Quest sales representative