Walnut Whole Allergen Testing

Test code 3489

Determine which of these proteins your patient has high levels of IgE to


One of the most common causes of allergic reactions to tree nuts1,2

  • Estimated prevalence of walnut allergy in the general population is up to 0.7%2
  • Potentially life-threatening, increasing in prevalence, and rarely outgrown2,3

Jug r1

Associated with systemic reactions2

  • Storage protein (2s albumin)3,4
  • Heat and digestion stable4
  • Highly abundant in walnuts

Jug r3

Associated with local and systemic reactions2

  • Lipid transfer protein (LTP)1,4
  • Heat and digestion stable

Positive whole walnut with negative Jug r1 and Jug r3 results may be explained by
sensitization to:6

  • Other walnut storage proteins
  • Pollen proteins like profilin or PR-10 proteins
  • Cross-reacting carbohydrate determinants (CCD)

As in all diagnostic testing, a diagnosis must be made by the physician based on test results, individual patient history, the physician’s knowledge of the patient, and the physician’s clinical judgement.

Get more information

For more information, contact your local Sales Representative.


For clinical information and specimen requirements,
visit the test directory.


Download the Tree Nut Allergen Component Testing brochure.


1. Roux K, et al. Tree nut allergens. Int Arch Allergy Immunology. 2003; 131: 234-244.

2. Pastorello E, et al. Lipid transfer protein and vicilin are important walnut allergens in patients not allergic to pollen. J Allergy Clin Immunol. 2004; 114(4): 908-914.

3. Rosenfeld L, et al. Walnut allergy in peanut-allergic patients: significance of sequential epitopes of walnut homologous to linear epitopes of Ara h 1, 2 and 3 in relation to clinical reactivity. Int Arch Allergy Immunol. 2012; 157:238-245.

4. Masthoff L, et al. A systematic review of the effect of thermal processing on the allergenicity of tree nuts. Allergy. 2013; 68: 983-993.

5. Egger M, et al. The role of lipid transfer proteins in allergic diseases. Curr Allergy Asthma Rep. 2010; 10:326-335.

6. www.phadia.com