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PAX8/PPAR[gamma] Translocation, Thyroid Cancer
Real-Time Polymerase Chain Reaction (PCR)
PAX8/PPARG translocation is associated with 25-50% of follicular thyroid carcinoma but has also been reported at low frequency in follicular adenomas. Correlation with morphologic and clinical findings is thus required. This assay detects elevated levels of PPARG transcripts, compared to a control gene, associated with PAX8/PPARG translocations by an RNA-based real-time quantitative reverse transcription PCR-based method. The lower limit of sensitivity is approximately 2.5% mutation-bearing cells in a mixed sample, but can vary due to RNA preservation and differing PPARG expression levels in any given neoplasm.
Follicular Thyroid Cancer
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