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Hepatitis B Virus DNA, Quantitative, Real-Time PCR

Test Code


CPT Code(s)


Preferred Specimen(s)

3 mL plasma collected in EDTA (lavender-top) tube

Minimum Volume

1 mL

Alternative Specimen(s)

Plasma collected in: PPT potassium EDTA (white-top) • Serum

Collection Instructions

Do not freeze whole blood or any samples stored in primary tube.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma or serum from cells within 24 hours of collection by centrifugation. Follow manufacturer's instructions for collection tube handling.

Transport Temperature


Specimen Stability

Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 84 days

Reject Criteria

Received room temperature


Real-Time Polymerase Chain Reaction

The analytical performance characteristics of this assay have been determined by Quest Diagnostics. The modifications have not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

Setup Schedule

Set up: Tues-Sat a.m.; Report available: Next day


Results obtained from immunosuppressed patients should be interpreted with caution.

Reference Range(s)

Hepatitis B Virus DNA Not Detected IU/mL
Hepatitis B Virus DNA Not Detected Log IU/mL

Clinical Significance

Chronic carriers will persist in producing detectable HBV. Patients with chronic liver disease of unknown origin most commonly have HBV that is detected by viral DNA testing. Quantitative measurement of HBV viral DNA may be used to monitor progression of disease.

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result NameLOINC CodeComponent Name
35222AHepatitis B Virus DNA42595-9Hepatitis B virus DNA
35222BHepatitis B Virus DNA48398-2Hepatitis B virus DNA

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.