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Ureaplasma urealyticum Culture
(Mycoplasma T Strain)

Test Code

5499

CPT Code(s)

87109

If culture is positive, an identification is performed at an additional charge (CPT: 87077 or 87140 or 87143 or 87147 or 87149).

Preferred Specimen(s)

Preferred Specimens: Urogenital (vaginal, cervical, urethral swabs or secretions); Respiratory (sputum, bronchial washing, trancheobronchial secretions, bronchoalveolar lavage, nasopharyngeal or throat swabs), sterile body fluids, tissue and wounds.

Swab of urogenital specimen, sterile fluid, tissue, or respiratory samples, submitted in VCM Transport Medium, refrigerated (cold packs)

Minimum Volume

1 mL

Alternative Specimen(s)

Random urine, semen, transtracheal aspirate (neonates), or CSF (neonates)

Urine pellet, submitted in VCM (or M4) Transport Medium, refrigerated (cold packs)
--or--
Swab of urogenital specimen, sterile fluid, tissue, or respiratory samples, submitted in M4 Transport Medium, refrigerated (cold packs)

Collection Instructions

Specimen Collection/Timing
Swab:
Collect specimen using a calcium-alginate swab, dacron, or polyester-tipped swab on a plastic or wire shaft. Place swab in a VCM Transport Tube and refrigerate.
Tissue:
Submit tissue specimen in a sterile cup with sufficient VCM Transport Medium to prevent drying, or place tissue in a VCM Transport Tube and refrigerate.
Fluid:
Submit fluid, including urine, in a sterile, leakproof container, or add 1-3 mL of specimen directly into a VCM Transport Tube and refrigerate.
Urine: 
Centrifuge at 3000 rpm for 15 minutes. Suspend sediment in VCM Transport. Use a 1:1 volume of urine in VCM Transport Tube if the specimen is not centrifuged.
M4 Transport Medium is also acceptable.

Specimen Container

See Instructions.

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: 30 days (-70ºC)

Reject Criteria

Unacceptable Specimens:
Specimens collected on wooden shafted swabs or cotton swabs;
Specimen received in expired transport medium;
Tissue specimen in Formalin;
Urine containing any preservatives.


Other Considerations: Ureaplasmas and mycoplasmas are extremely sensitive to adverse environmental conditions, particularly drying, osmotic changes, toxic metabolites, and temperature fluctuations.

Methodology

Culture

Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

Setup Schedule

Daily a.m., p.m., and night shift; Report available: 10 days

Reference Range(s)

No Ureaplasma isolated

Clinical Significance

Ureaplasma urealyticum and Mycoplasma hominis are primarily associated with genital tract colonization and disease in adults and respiratory tract colonization and disease in newborns. Though controversial, these organisms have been associated with endometritis, chorioamnionitis, premature rupture of membranes, stillbirth, premature birth, low birth weight, postpartum infections, and infertility. Of particular concern is the causual relationship between central nervous system infections in the premature newborn and U. urealyticum. The serious complications of disease associated with Ureaplasma and Mycoplasma infections have led to an increased demand for laboratory identification of these organisms. The extremely small amount of genetic material possessed by these organisms makes them demanding in their nutritional requirements for cultivation.

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.