Vitamin B1 (Thiamine), Blood LC/MS/MS

CPT Code(s)

84425

Patient Preparation

Overnight fasting is recommended

Preferred Specimen(s)

3 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

1 mL

Collection Instructions

Transfer whole blood to a plastic shipping vial to prevent breakage. Wrap tube in aluminum foil to protect from light. Freeze immediately.

Samples received frozen but not protected from light are acceptable. Wrap sample in foil before sending to lab, no disclaimer is required. Samples received refrigerated but not protected from light are unacceptable and should be rejected.

Transport Temperature

Frozen

Specimen Stability

Room temperature: 72 hours

Refrigerated: 5 days

Frozen: 5 weeks

Reject Criteria

Thawed whole blood • Received room temperature • Received refrigerated

Methodology

Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Performing Laboratory

Quest Diagnostics Nichols Institute

33608 Ortega Highway

San Juan Capistrano, CA 92690-6130

Setup Schedule

Set up: Sun-Thurs; Report Available: 5-8 days



This assay has an overnight incubation period

Reference Range(s)

Adults: 78-185 nmol/L

Clinical Significance

Vitamin B1 is required for branched-chain amino acid and
carbohydrate metabolism. Vitamin B1 deficiency is most
often due to alcoholism or chronic illness. In the early
stage, patients with Vitamin B1 deficiency exhibit
anorexia, irritability, apathy, and generalized weakness.
Prolonged deficiency causes beriberi.