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CULTURE, ANAEROBIC BACTERIA W/GRAM STAIN

Test Code

4469

CPT Code(s)

87075
87205

Preferred Specimen(s)

3 ML OR 3 GRAMS ABSCESS OR DEEP WOUND, TISSUE, NORMALLY
STERILE BODY FLUIDS OTHER THAN BLOOD, BRONCHOSCOPIC
SPECIMENS OBTAINED BY THE DOUBLE LUMEN BRUSH TECHNIQUE,
SUPRAPUBIC URINE ASPIRATES OBTAINED FROM BLADDER,
NEPHROSTOMY TUBE OR SUPRAPUBIC CATHETERIZATION, ASPIRATED
PUS FROM DEEP WOUND OR ABSCESS OR TYMPANOCENTESIS FLUID,
GENITAL SPECIMENS FROM THESE SITES ONLY: CUL-DE-SAC
ASPIRATION, CULDOCENTESIS-PERCUTANEOUS TRANSFUNDAL
ASPIRATION, FALLOPIAN TUBE, SURGICAL SPECIMENS, EXUDATES,
COLLECTED IN PORT-A-CUL(TM) GEL VIAL

INSTRUCTIONS:
TISSUES AND FLUIDS ARE SUPERIOR TO A SWAB SPECIMEN. IF
SWABS MUST BE USED, COLLECT 2 ANAEROBIC TRANSPORT SWABS,
ONE FOR CULTURE AND ONE FOR GRAM STAIN OR SUBMIT ONE
ANAEROBIC TRANSPORT SWAB WITH ONE AIR DRIED SMEAR OR ONE
ESWAB (WHITE-CAP) FOR BOTH CULTURE AND GRAM STAIN. SPECIMENS
NOT LISTED AS ACCEPTABLE MAY BE PROCESSED FOR ANAEROBIC
CULTURE ONLY AFTER CONSULTATION WITH A QUEST MICROBIOLOGY
SCIENTIFIC DIRECTOR. DO NOT SUBMIT SYRINGE WITH NEEDLE
ATTACHED. IF SUBMITTING SYRINGE, REMOVE NEEDLE, EXPEL AIR
AND CAP SYRINGE. PURULENT STERILE BODY FLUIDS NOT SUBMITTED
IN AN ANAEROBIC TRANSPORT TUBE MAY BE ACCEPTED FOR CULTURE
IF RECEIVED WITHIN 8 HOURS OF COLLECTION.

Minimum Volume

1 ML OR 1 GRAM

Alternative Specimen(s)

(2 SWABS) ABSCESS OR DEEP WOUND, NORMALLY STERILE BODY
FLUIDS OTHER THAN BLOOD, BRONCHOSCOPIC SPECIMENS OBTAINED
BY THE DOUBLE LUMEN BRUSH TECHNIQUE, SUPRAPUBIC URINE
ASPIRATES OBTAINED FROM BLADDER, NEPHROSTOMY TUBE OR
SUPRAPUBIC CATHETERIZATION, ASPIRATED PUS FROM DEEP WOUND
OR ABSCESS OR TYMPANOCENTESIS FLUID, GENITAL SPECIMENS FROM
THESE SITES ONLY: CUL-DE-SAC ASPIRATION, CULDOCENTESIS-
PERCUTANEOUS TRANSFUNDAL ASPIRATION, FALLOPIAN TUBE,
SURGICAL SPECIMENS, EXUDATES, COLLECTED IN AMIES GEL (BLUE-
CAP) OR ESWAB (WHITE-CAP)

Transport Container

PORT-A-CUL(TM) GEL VIAL FOR FLUID AND TISSUE SPECIMENS
ANAEROBIC TRANSPORT SWAB WITH AIMES GEL (BLUE-CAP) OR
ESWAB (WHITE-CAP)

Transport Temperature

REFRIGERATED (COLD PACKS)

Specimen Stability

PORT-A-CUL(TM):
ROOM TEMPERATURE: 72 HOURS
REFRIGERATED: 72 HOURS
FROZEN: UNACCEPTABLE

ESWAB OR SWAB IN AMIES:
ROOM TEMPERATURE: 48 HOURS
REFRIGERATED: 48 HOURS
FROZEN: UNACCEPTABLE

Reject Criteria

SPECIMENS NOT SUBMITTED IN ANAEROBIC TRANSPORT MEDIA/DEVICE
EXPIRED TRANSPORT MEDIA
THROAT, NASOPHARYNGEAL, SPUTUM OR BRONCHOSCOPIC SPECIMENS
SPECIMENS NOT COLLECTED BY DOUBLE LUMEN BRUSH TECHNIQUE
STOOL AND RECTAL SWABS
URINE-VOIDED OR CATHETER
VAGINAL AND CERVICAL
SPECIMENS FROM SITES CONTAMINATED WITH INTESTINAL CONTENTS
SUPERFICIAL OR FRESH WOUNDS
EXTERNAL EAR
EXTERNAL EYE
INTRAUTERINE DEVICE
TRACHEAL ASPIRATES

Methodology

DIRECT MICROSCOPY; BACTERIAL CULTURE; ANAEROBIC ISOLATION
AND IDENTIFICATION PROCEDURES: PHENOTYPIC; MATRIX ASSISTED
LASER DESORPTION IONIZATION-TIME OF FLIGHT; SEQUENCING

Performing Laboratory

DIAGNOSTIC LABORATORY OF OKLAHOMA
225 NE 97TH STREET
OKLAHOMA CITY OK 73114-6302

Clinical Significance

ANAEROBIC BACTERIA CAUSE A VARIETY OF HUMAN INFECTIONS.
THE RESULTS OF ANAEROBIC CULTURES ASSIST THE CLINICIAN
WITH DIAGNOSIS AND TREATMENT OF PATIENTS WITH ANAEROBIC
INFECTIONS.

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.