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Culture, Blood

Test Code


CPT Code(s)



Aerobic culture, anaerobic culture. If culture is positive, identification will be performed at an additional charge (CPT code(s): 87076 or 87106 or 87077 or 87140 or 87143 or 87147 or 87149).
Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87181 or 87184 or 87185 or 87186).

Preferred Specimen(s)

Adults: 8-10 mL of blood in each of BD BACTEC™ Plus™ Aerobic/F (blue label and cap) and Lytic/10 Anaerobic/F bottles (purple label and cap)

Neonates and children 1 to 6 years: 1-3 mL of blood in a BACTEC™ Peds Plus™/F bottle

Children weighing 30-80 lbs: 3 mL of blood in BD BACTEC™ Peds Plus™/F bottle or 8-10 mL of blood in BD BACTEC™ Plus™ Aerobic/F and Lytic/10 Anaerobic/F bottles

See the alphabetical listing in the Microbiology section of Specimen Collection and Handling for further instructions.

Minimum Volume

BACTEC™ Plus™ Aerobic/F and Lytic/10 Anaerobic/F bottles: 3 mL blood/bottle • BACTEC™ Peds Plus™/F bottles: 0.5 mL blood/bottle is acceptable, but 1 mL is preferred.

Alternative Specimen(s)

Bone marrow cultures: For adults, use 1 BACTEC™ Plus™ Aerobic/F and 1 BACTEC™ Lytic/10 Anaerobic/F bottles. For children, use a BACTEC™ Peds Plus™ Aerobic/F bottle for aerobic and anaerobic testing • Blood culture bottles compatible with local automated instrumentation.

Collection Instructions

Do not pre-incubate bottles prior to shipment

Transport Container

BD BACTEC™ Plus™ Aerobic/F (blue label and cap) and Lytic/10 Anaerobic/F bottles (purple label and cap), or BD BACTEC™ Peds Plus™/F

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria

Blood specimens collected in EDTA, sodium citrate or anticoagulants such as SPS or heparin • Frozen specimens • Specimens broken in transit


Automated continuous monitoring using fluorescent detection. Antibiotic susceptibility testing when appropriate.

Performing Laboratory

Quest Diagnostics - Great Lakes Region

Setup Schedule

Set up: Daily; Report available: 3 days

Clinical Significance

Bacterial sepsis constitutes one of the most serious infectious diseases. The detection of microorganisms in a patient's blood has importance in the diagnosis and prognosis of endocarditis, septicemia, or chronic bacteremia.

Alternative Name(s)

Listeria Culture, Fungus Culture, Blood, Blood Culture, Brucella, Blood Culture

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result NameLOINC CodeComponent Name
75400008CULTURE, BLOOD600-7Bacteria identified
75400009CULTURE600-7Bacteria identified
75400010ID, METHOD NOT SPECIFIED- (AER)BIO,COAG,VTK,API23667-9Bacteria identified
75400012ID BY GLC23667-9Bacteria identified
75400013ID BY PROBE23667-9Bacteria identified
75400026SEROLOGICAL GROUPING (BETA STREP)612-2Bacteria identified
75400027BETA LACTAMASE20423-0Beta lactamase organism identified
75400028MIC SENSITIVITY (BREAK POINT)21070-8Antibiotic XXX
75400029KIRBY-BAUER (DISK DIFFUSION)45187-2Antibiotic XXX
75400030E-TEST55617-5Antibiotic XXX
75400417SALMONELLA/SHIGELLA ID/TYPING612-2Bacteria identified
75400561FLUORESCENT ID, EACH (i.e. GONOCOCCUS)35347-4Microscopic observation
75400641SOURCE:31208-2Specimen source
75407856DEFINITIVE ID, YEAST42804-5Fungus identified

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.