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HEXAGONAL PHASE CONFIRMATION

Test Code

36573X

CPT Code(s)

85598

Preferred Specimen(s)

Preferred Specimen Volume: 2 ml
Specimen Type CITRATED PLASMA (BLUE TOP TUBE)
Specimen State FROZEN
Hemolyzed specimens are not acceptable.
3.2% Sodium citrate (lt.blue-top) preferred.

Please submit a separate, frozen vial for each special
coagulation assay ordered.

Freshly collected platelet poor plasma is required. Use
only siliconized or plastic collection tube to collect
blood (follow NCCLS guidelines H3-A3 and H21-A2).

An important step in the diagnosis of the lupus
anticoagulant (LA) is appropriate specimen collection and
processing. The more platelet-free the sample, the greater
the sensitivity of most test systems to the presence of LA.
It is imperative for the laboratory to take precautions in
preparing platelet poor plasma (PPP).

Ideally, PPP should have a platelet count of less than 10 x
109 per liter (<10,000/uL). Although the sample collection
process described in the Coagulation Specimens in Specimen
Collection section of Test Dictionary should yield PPP,
the following double-spin technique can also be used:

1. Spin down specimen at 1500 x g for 15 minutes.
2. Transfer the plasma to a plastic tube with a plastic
Pasteur pipette, staying away from the buffy coat layer.
Spin down the plasma portion again at 1500g for 15
minutes. With another plastic Pasteur pipette, transfer
the plasma to another plastic tube, staying clear of the
bottom of the tube where platelets lie.
Alternately, the plasma may be filtered using a 0.2 micron
filter.
3. Transfer plasma into a plastic tube using a plastic
Pasteur pipette. Do not use glass tubes or glass Pasteur
pipettes, as glass can activate the clotting cascade.
4. Label each tube plasma. Submit a plasma aliquot for
each and every coagulation assay requested (one tube for
each test). If possible, submit one additional plasma
aliquot for repeat and/or test additions.

STABILITY:
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 90 days

Minimum Volume

1 ml

Transport Container

Blue - Sodium Citrate

Methodology

CLOTTING ASSAY

Performing Laboratory

907-CHANTILLY,NICHOLS QUEST DIAGNOSTICS

Setup Days

M,T,W,TH,F

Report Available

2 DAYS

Reference Range(s)

36573X HEXAGONAL PHASE CONFIRMATION (CHANTILLY,NICHO
CONSTITUENTS [LOINC] UNITS SEX/HI AGE NORMALS
==================== ===== ========== =======
Z8937 [339309] HEXAGONAL PHASE CONFIRMATION

Clinical Significance

Hemolyzed specimens are not acceptable.
3.2% Sodium citrate (lt.blue-top) preferred.

Please submit a separate, frozen vial for each special
coagulation assay ordered.

Freshly collected platelet poor plasma is required. Use
only siliconized or plastic collection tube to collect
blood (follow NCCLS guidelines H3-A3 and H21-A2).

An important step in the diagnosis of the lupus
anticoagulant (LA) is appropriate specimen collection and
processing. The more platelet-free the sample, the greater
the sensitivity of most test systems to the presence of LA.
It is imperative for the laboratory to take precautions in
preparing platelet poor plasma (PPP).

Ideally, PPP should have a platelet count of less than 10 x
109 per liter (<10,000/uL). Although the sample collection
process described in the Coagulation Specimens in Specimen
Collection section of Test Dictionary should yield PPP,
the following double-spin technique can also be used:

1. Spin down specimen at 1500 x g for 15 minutes.
2. Transfer the plasma to a plastic tube with a plastic
Pasteur pipette, staying away from the buffy coat layer.
Spin down the plasma portion again at 1500g for 15
minutes. With another plastic Pasteur pipette, transfer
the plasma to another plastic tube, staying clear of the
bottom of the tube where platelets lie.
Alternately, the plasma may be filtered using a 0.2 micron
filter.
3. Transfer plasma into a plastic tube using a plastic
Pasteur pipette. Do not use glass tubes or glass Pasteur
pipettes, as glass can activate the clotting cascade.
4. Label each tube plasma. Submit a plasma aliquot for
each and every coagulation assay requested (one tube for
each test). If possible, submit one additional plasma
aliquot for repeat and/or test additions.

STABILITY:
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 90 days

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result
Code
Result NameLOINC CodeComponent Name
Z8937HEXAGONAL PHASE CONFIRMATION33930-9Lupus anticoagulant neutralization.hexagonal phase phospholipid

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.