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dRVVT Screen with Reflex to dRVVT Confirm and dRVVT 1:1 Mix [15780X]
If dRVVT Screen is prolonged (>45 seconds), dRVVT Confirm will be performed at an additional charge (CPT code(s): 85597).
If dRVVT Confirm is positive, dRVVT 1:1 Mixing Study will be performed at an additional charge (CPT code(s): 85613).
2 mL frozen platelet-poor plasma collected in a 3.2% sodium citrate (light blue-top) tube
Please submit a separate, frozen vial for each special coagulation assay ordered.
Platelet Poor Plasma for Lupus Anticoagulant Testing: Perhaps the most important step in the diagnosis of the lupus anticoagulant (LA) is appropriate specimen collection and processing. It is imperative that the laboratory take extra precautions in preparing platelet-poor plasma (PPP). The more platelet-free the sample, the greater the sensitivity of most test systems to the presence of LA. Ideally, PPP should have a platelet count of less than 10 x 109 per liter (<10,000/mcL). Although the sample collection process described in the Coagulation Specimens in the Specimen Collection section of Test Directory should yield PPP, the following double-spin technique can also be used:
1. Spin down specimen at 1500 x g for 15 minutes.
2. Transfer the plasma to a plastic tube with a plastic Pasteur pipette, staying away from the buffy coat layer. Spin down the plasma portion again at 1500 x g for 15 minutes. With another plastic Pasteur pipette, transfer the plasma to another plastic tube, staying clear of the bottom of the tube where the platelets lie. Alternatively, the plasma may be filtered using a 0.2 micron filter.
3. Transfer plasma into a plastic tube using a plastic Pasteur pipette. Do not use glass tubes or glass Pasteur pipettes, as glass can activate the clotting cascade.
4. Label each tube plasma. Submit a plasma aliquot for each and every coagulation assay requested (one tube for each test). If possible, submit one additional plasma
aliquot for repeat and/or test additions.
Plastic screw-cap vial
Room temperature: Unacceptable
Frozen: 90 days
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
Set up: Mon-Sat (night shift).; Report available: Next day
|dRVVT Screen||≤45 seconds|
|dRVVT 1:1 Mix||Corrected|
Diagnosis of a lupus anticoagulant (LA) requires testing with a least 2 screening reagents, followed by confirmatory testing. The dRVVT screen, confirm, and mix satisfy international guidelines for LA evaluations.
The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.NOTE: The codes listed in the table below are not orderable Test Codes.
|Result Name||LOINC Code||Component Name|
|33693C||dRVVT 1:1 Mix||34569-4||Coagulation dilute Russell viper venom induced.factor substitution^immediately after 1:2 addition of normal plasma|
|3369A||dRVVT Screen||6303-2||Coagulation dilute Russell viper venom induced|
|3369C||dRVVT||6303-2||Coagulation dilute Russell viper venom induced|
|3369I||dRVVT Mix Interpretation:||50008-2||Mixing studies|
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.
* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.