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Acanthamoeba/Naegleria Culture

Test Code


CPT Code(s)


Preferred Specimen(s)

1 mL CSF collected in a CSF collection tube • 3 mm biopsy or equivalent piece of tissue from brain, lung, cornea, or lesion; corneal scrapings from affected area; 1 swab of conjunctiva/corneal ulcer • Both contact lenses or lens care solution from an open container already in use by patient

Minimum Volume

0.5 mL

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria

Sources not listed • Specimens received dry • Received refrigerated or frozen • Specimens in transport media • Liver fluid • Slides • Swabs in bacterial or viral transport medium



Performing Laboratory

Quest Diagnostics Infectious Disease, Inc.
33608 Ortega Highway, Bldg B-West Wing
San Juan Capistrano, CA 92675-2042

Setup Schedule

Set up: Daily; Report available: 11-12 days

Reference Range(s)

Not isolated

Clinical Significance

Small free-living amebae belonging to the genera Acanthamoeba and Naegleria have been identified as agents of human disease, causing infections of the central nervous system, cornea, lungs, and skin. These amebae are commonly found in soil, fresh water, sewage, and sludge. Acanthamoeba spp. are known to cause Chronic Granulomatous Amebic Encephalitis as well as a painful vision-threatening keratitis. Naegleria Fowleri is implicated in primary Amebic Meningoencephalitis, an acute fulminating disease that is often fatal. This test is for the culture of Acanthamoeba and Naegleria only. For detection of intestinal amebae, such as Entamoeba Histolytica, please request Focus unit code 51730, Ova and Parasite Examination.

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result NameLOINC CodeComponent Name
75000000Source31208-2Specimen source
75005300Amoeba Culture6594-6Amoeba identified

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.