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Testosterone, Free

Test Code


CPT Code(s)


Preferred Specimen(s)

2.8 mL serum collected in a red-top tube (no gel)

Minimum Volume

1.3 mL

Collection Instructions

Please specify age and sex on test request form.

Specimen Container

Plastic screw-cap vial

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: 2 years

Reject Criteria

Gross hemolysis • Gross lipemia • Serum Separator Tube (SST®)


Chromatography/Mass Spectrometry • Calculation (CALC) • Immunochemiluminescence Assay (ICMA)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

Setup Schedule

Set up: Mon-Fri a.m.; Report available: 2 days

Reference Range(s)

  Male (pg/mL) Female (pg/mL)
<1 Year Not established Not established
1-11 Years ≤1.3 ≤1.5
12-13 Years ≤64.0 ≤1.5
14-17 Years 4.0-100.0 ≤3.6
18-69 Years 46.0-224.0 0.2-5.0
70-89 Years 6.0-73.0 0.3-5.0
>89 Years Not established Not established

Clinical Significance

Test is helpful in assessing testicular function in males and managing hirsutism and virilization in females.

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result NameLOINC CodeComponent Name
15983Testosterone, Total, MS2986-8Testosterone
30740Sex Hormone Binding Glob13967-5Sex hormone binding globulin

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.