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Vitamin D, 25-Hydroxy, LC/MS/MS
Vitamin D, 25-OH, Total; Vitamin D, 25-OH, D3; Vitamin D, 25-OH, D2
Fasting preferred, but not required
0.5 mL serum
Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a Serum Separator Tube (SST®), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.
Plastic screw-cap vial
Room temperature: 21 days
Refrigerated: 21 days
Frozen -20°C: 21 days (glass tubes unacceptable)
Frozen -70°C: 18 months (glass tubes unacceptable)
Gross hemolysis • Gross lipemia • Heparinized or EDTA plasma • Serum not separated from Serum Separator Tube (SST®) gel or clot within 48 hours
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
|Quest Diagnostics Nichols Institute|
|14225 Newbrook Drive|
|Chantilly, VA 20153|
Set up: Mon-Fri Evening; Report available: 3 days
|Vitamin D, 25-OH, Total||30-100 ng/mL|
|Vitamin D, 25-OH, D3||Not established|
|Vitamin D, 25-OH, D2||Not established|
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25OH D3 - the endogenous form of the vitamin and 25OH D2 - the analog form used to treat 25OH Vitamin D3 deficiency.
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
(The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.)
* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.