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ANA IFA, with Reflex to Titer/Pattern/Cascade
The ANA Screen,IFA, Reflex Titer/Pattern,and Reflex to Multiplex 11 Ab Cascade begins with an ANA Screen, IFA.
If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).
Additionally, five antibodies will be performed at an additional charge: dsDNA (CPT code(s): 86225), Sm/RNP (CPT code(s): 86235), RNP (CPT code(s): 86235), Sm (CPT code(s): 86235), and Chromatin (CPT code(s): 86235).
If any of those five antibodies are positive, the cascade stops and the results are reported.
If all five of those antibodies are negative, four additional antibodies will be performed at an additional charge: SSA (CPT code(s): 86235), SSB (CPT code(s): 86235), Scl-70 (CPT code(s): 86235), Jo-1 (CPT code(s): 86235).
If any of those four antibodies are positive, the cascade stops and the results are reported.
If all four of those antibodies are negative, the following two additional antibodies will be performed at an additional charge: Ribosomal P (CPT code(s): 83516) and Centromere B (CPT code(s): 86235).
Please note the cascade stops upon the first positive antibody result(s) found in a group and an interpretive message is applied based on this information. It is possible that antibodies in subsequent groups are also positive, but will not be added, billed, or reported. Please contact your local Quest Diagnostics Laboratory if you are interested in adding this additional testing.
1 mL serum
Plastic screw-cap vial
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 30 days
Gross hemolysis • Grossly lipemic • Grossly icteric • Microbial contamination may interfere
Immunofluorescent Assay (IFA)
|Quest Diagnostics Nichols Institute|
|14225 Newbrook Drive|
|Chantilly, VA 20153|
Set up: Mon-Sat a.m.; Report available: Next day
|1:40-1:80||Low antibody level|
|>1:80||Elevated antibody level|
Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE.
Mixed Connective Tissue Disease, MCTD
The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.NOTE: The codes listed in the table below are not orderable Test Codes.
|Result Name||LOINC Code||Component Name|
|132812||Sm/RNP Antibody||14030-1||Smith extractable nuclear Ab+Ribonucleoprotein extractable nuclear Ab|
|132822||Sm Antibody||43182-5||Smith extractable nuclear Ab|
|137032||Chromatin (Nucleosomal) Ab||34416-8||Nucleosome Ab|
|18167A||ANA Titer||5048-4||Nuclear Ab|
|18167B||ANA Pattern||13068-2||Nuclear Ab pattern|
|19887||RNP Antibody||29374-6||Ribonucleoprotein extractable nuclear Ab|
|38318A||ANA Screen, IFA||42254-3||Nuclear Ab|
|5962||DNA (ds) Antibody||5130-0||DNA double strand Ab|
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.
* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.