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Vitamin D, 1,25-Dihydroxy, LC/MS/MS

Test Code


CPT Code(s)



Vitamin D,1,25 (OH)2,Total; Vitamin D3, 1,25 (OH)2; Vitamin D2, 1,25 (OH)2

Preferred Specimen(s)

1 mL serum

Minimum Volume

0.5 mL

Collection Instructions

Collect blood in a Vacutainer®. Allow blood to clot (30 minutes) at room temperature, 18-25° C. Centrifuge and separate the serum from the cells.

Note: If sample is submitted with less than 1.1 mL and needs to be repeated the sample will be cancelled with the comment "TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat".

Specimen Container

Plastic screw-cap vial

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 28 days
Refrigerated: 28 days
Frozen: 28 days

Reject Criteria

Gross hemolysis • lipemia • Icteric specimens • plasma


Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

Setup Schedule

Set up: Mon-Sat evening; Report available: 3 days

Reference Range(s)

Vitamin D, 1,25 (OH)2, Total  
1-9 Years 31-87 pg/mL
10-13 Years 30-83 pg/mL
14-17 Years 19-83 pg/mL
 Adult 18-72 pg/mL
Vitamin D3, 1,25 (OH)2 Not available
Vitamin D2, 1,25 (OH)2 Not available
Reference ranges are established for total 1,25-dihydroxy vitamin D. Values for subcomponents D2 (derived from plant or fungal sources) and D3 (derived from human or animal sources) are provided for informational purposes only.

Clinical Significance

This test measures the bioactive form of vitamin D. It is used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure. This test is not suitable for diagnosis of vitamin D deficiency and monitoring supplementation in most patients. The 25-hydroxyvitamin D test is the recommended test for those purposes (N Engl J Med. 2007;357:266-281).

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result NameLOINC CodeComponent Name
86003320Vitamin D,1,25 (OH)2,Total62290-21,25-Dihydroxyvitamin D
86003321Vitamin D3, 1,25 (OH)21649-3Calcitriol
86003322Vitamin D2, 1,25 (OH)262291-01,25-Dihydroxyvitamin D2

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.