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Vitamin D, 1,25-Dihydroxy, LC/MS/MS

Test Code

16558

CPT Code(s)

82652

Includes

Vitamin D,1,25 (OH)2,Total; Vitamin D3, 1,25 (OH)2; Vitamin D2, 1,25 (OH)2

Preferred Specimen(s)

2 mL serum

Minimum Volume

1.1 mL

Collection Instructions

Collect blood in a Vacutainer®. Allow blood to clot (30 minutes) at room temperature, 18-25° C. Centrifuge and separate the serum from the cells.
If sample is submitted with less than 1.1 mL and needs to be repeated the sample will be canceled with the comment "TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat".

Specimen Container

Plastic screw-capped vial

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 28 days
Refrigerated: 28 days
Frozen: 28 days

Reject Criteria

Grossly hemolyzed • Lipemic • Icteric specimens • Plasma samples

Methodology

Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

This test was developed and its performance characteristics have been determined by Quest Diagnostics Nichols Institute. Performance characteristics refer to the analytical performance of the test.

Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

Setup Schedule

Set up: Mon-Sat evening; Report available: 4 days

Reference Range(s)

Vitamin D, 1,25 (OH)2, Total  
1-9 Years 31-87 pg/mL
10-13 Years 30-83 pg/mL
14-17 Years 19-83 pg/mL
 Adult 18-72 pg/mL
Vitamin D3, 1,25 (OH)2 Not available
Vitamin D2, 1,25 (OH)2 Not available
Reference ranges are established for total 1,25-dihydroxy vitamin D. Values for subcomponents D2 (derived from plant or fungal sources) and D3 (derived from human or animal sources) are provided for informational purposes only.

Clinical Significance

This test is used to measure the bio-active form of Vitamin D. This test is also used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure.

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result
Code
Result NameLOINC CodeComponent Name
86003320Vitamin D,1,25 (OH)2,Total62292-825-Hydroxyvitamin D2+25-Hydroxyvitamin D3
86003321Vitamin D3, 1,25 (OH)21649-3Calcitriol
86003322Vitamin D2, 1,25 (OH)262291-01,25-Dihydroxyvitamin D2

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.