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ACTH Stimulation, 3 Specimens [14930X]

Test Code


CPT Code(s)

82533 (x3)


Baseline, 30 minutes post ACTH administration and 60 minutes post ACTH administration

Preferred Specimen(s)

1 mL (x3) serum

Minimum Volume

0.5 mL (x3)

Collection Instructions

Draw baseline specimen before ACTH administration. Administer 250 mcg Cortrosyn by rapid IV push. Draw specimens 30 and 60 minutes after ACTH administration. Clearly mark time drawn on all tubes.

Note: Cortrosyn (Cosyntropin) is not supplied by the laboratory.

Collect 1 mL serum for each timed sample from red-top tubes (no gel), or from serum separator tubes (SST®). Centrifuge and transfer serum specimens to (x3) separate sterile, plastic screw-capped vials. Transport (x3) samples at room temperature in the same shipping container.

Specimen Container

Plastic screw-cap vial(s)

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days


Immunoassay (IA)

Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

Setup Schedule

Set up: Daily; Report available: Next day

Reference Range(s)

See Laboratory Report

Clinical Significance

Test for adrenal function/Addison's Disease.

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result NameLOINC CodeComponent Name
149308ACortisol 312557-5Cortisol^2nd specimen post XXX challenge
67348ATime 119151-0Specimen drawn
67348BCortisol 150453-0Cortisol^1st specimen
67348CTime 219151-0Specimen drawn
67348DCortisol 250454-8Cortisol^2nd specimen
67348ETime 319151-0Specimen drawn

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.