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Cortisol, Free, 24-Hour Urine with Creatinine, LC/MS/MS

Test Code


CPT Code(s)


Preferred Specimen(s)

2 mL urine from a 24-hour collection (no preservatives)

Minimum Volume

0.5 mL

Alternative Specimen(s)

25 mL 6N HCl • 10 mL conc.  glacial acetic acid • 10 g boric acid

Collection Instructions

Record 24-hour urine volume on test requisition and urine vial. Reference ranges do not apply to random urine samples.

Transport Container

Transport tube

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 5 months


Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

Setup Schedule

Set up: Mon-Sat a.m.; Report available: 4 days

Clinical Significance

Urinary Free Cortisol is useful in the detection of patients with Cushing's syndrome for whom Free Cortisol concentrations are elevated.

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result NameLOINC CodeComponent Name
85986150TOTAL VOLUME3167-4Specimen volume
85986152CREATININE, URINE2162-6Creatinine
85987390CORTISOL, FREE, URINE2147-7Cortisol.free

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.