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To view specimen requirements and codes please select your laboratory:

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Test Code


CPT Code(s)


Preferred Specimen(s)

Whole blood from an EDTA (lavender-top) tube

Whole blood collected in an ACD Solution B (yellow-top)
tube is also acceptable. Cord blood specimens are also

If this test is submitted with a CBC, Hemoglobin A1C or
any other test requiring an EDTA (lavender-top) tube,
please submit a separate tube for this request.

Minimum Volume

3 mL

Specimen Stability

Room temperature: 3 days
Refrigerated: 6 days
Frozen: Unacceptable


Immune Agglutination

Performing Laboratory

Quest Diagnostics, Horsham

Setup Days

Set Up: Mon-Fri a.m.; Report available: 2 days

Reference Range(s)


Clinical Significance

The DAT (Direct Coomb's test) is positive if red cells have been coated, in vivo, with immunoglobulin, complement, or both. A positive result can occur in immune-mediated red cell destruction, autoimmune hemolytic anemia, a transfusion reaction or in patients receiving certain drugs.

LOINC®' Code(s)

The Result and LOINC information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table below are not orderable Test Codes.

Result NameLOINC CodeComponent Name
3520COOMBS,DIRECT,POLYSPECIFIC1007-4Direct antiglobulin test.poly specific reagent

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

LOINC assignment is based on a combination of test attributes, including the method used by the performing laboratory. For tests not performed by Quest Diagnostics, codes are assigned by the performing laboratory.

* The tests listed by specialist are a select group of tests offered. For a complete list of Quest Diagnostics tests, please refer to our Directory of Services.